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March 26, 2013
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Retention Time Variability in HPLC
A number of retention time issues can cause problems in HPLC. Here's advice on how to handle common ones, such as retention time drift, temperature variability, problems arising from column equilibration, and difficulties validating a  method.
Succeed from Sample Prep to Preparation
Agilent offers a complete portfolio of Fast LC columns—conventional HPLC or UHPLC and biocolumns in reversed phase, size exclusion, ion exchange and affinity—plus the sample prep chemistries you need to perfect your analysis. Bring your separations together with Agilent LC tools and resources at
Featured News

Biomarker for River Blindness Discovered
Scientists at The Scripps Institute (La Jolla, California) have used liquid chromatography–mass spectrometry (LC–MS) to identify a new biomarker for the diagnosis of river blindness. The discovery is an important step towards the development of a portable diagnostic test for use in the field.
Accelerate Your Productivity
Agilent Training is your fastest path to productivity.  From basic theory and practice to advanced operation, Agilent offers a variety of training courses to help you get ahead.  Whether you’re looking to train a new operator, or gain the expertise to expand the use of your instruments to new applications, Agilent Training is your best choice. View the new 2013 Agilent Training Catalog.

AB Sciex and Leco Partner to Provide Solutions for Metabolomics Researchers
AB Sciex (Framingham, Massachusetts) and Leco (St. Joseph, Michigan) have formed a co-marketing partnership to provide support for researchers working in metabolomics in Europe and North America.
5 Years Free Access to CHROMacademy
To show our commitment to learning, we have decided to offer all university students and staff free access to CHROMacademy.  You are the future leaders and innovators of the scientific community, and by offering free access to CHROMacademy we hope to help you realize your potential.  This has been made possible by Agilent Technologies- the world’s premiere measurement company. Learn more.

 LC Troubleshooting
Need Help With LC Troubleshooting?

A free interactive LC Troubleshooter is now available at CHROMacademy, sponsored by Thermo Scientific. In 3 simple steps you will overcome your instrument, separation and quantitation issues. It's completely free. More.

Featured Product
Active Flow Splitting Made Easy!
Rheodyne® programmable unit splits the flow stream in ratios ideal for LC/MS applications Active splitting within ±2% Reliably constant over time Compact stand-alone unit integrates easily with existing systems. Learn more
Advance Your Protein Precipitation
Phree Phospholipid Removal Plates offer a fast effective way to remove proteins AND phospholipids from plasma samples without negatively affecting the recovery of your target analytes.  No method development is required, simply add your sample and organic solvent to the Phree plate, mix, and filter! Learn more.
Nexera X2 UHPLC
The next milestone in the evolution of HPLC, Shimadzu’s Nexera X2 Series delivers superior, real-world performance across a wider application range while delivering unparalleled flexibility and reliability. Learn more.
First Refractive Index Detector for UHPLC
Wyatt Technology announces the immediate availability of the Optilab UT-rEX (UHPLC refractometer with EXtended range), the first RI detector specifically designed for use with UHPLC systems using columns packed with small beads. Using a combination of miniaturized components and cutting-edge semiconductor photodiode technology as well as proprietary computer algorithms, the UT-rEX presents a host of instrument firsts. Read more.
Market Profile: Automated DNA Sequencers
Automating the DNA sequence process is made possible by the use of fluorescent tags. With fluorescent dyes, the entire experiment can be performed in a single solution instead of four different ones.
New Webcasts
Ion Exchange Mechanisms in SPE: The Ultimate in Selectivity
Wednesday, April 3, 2013
11:00 AM EDT.
Editors' Series: Industrial Problem Solving Using Ion Chromatography
Wednesday, April 24, 2013
8:00 AM PDT; 11:00 AM EDT.
This web seminar will highlight the importance of IC for industrial problem solving and provide insights into some practical approaches used. Newer applications for IC in the area of total heteroatom determination using combustion-IC will also be introduced.
Upcoming Events

1–5 April 2013
2013 MRS Spring Meeting & Exhibit
San Francisco, CA
» Visit the website
1–5 April 2013
GMA (Grocery ManufacturersAssociation) Science Forum 2013
Washington, DC
» Visit the website
3–4 April 2013
Isocyanates and Health: Past, Present and Future
Potomac, MD
» Visit the website
7–11 April 2013
245th ACS National Meeting & Exposition
New Orleans, LA
» Visit the website
8–9 April 2013
5th Berlin LC/MSMS Symposium
Berlin, Germany
» Visit the website
8–11 April 2013
CFABS: 7th Workshop on Recent Issues in Bioanalysis(WRIB)
Long Beach, CA
» Visit the website
10–12 April 2013
15th International Trade Fair of Analytical, Measurement and Control Technology (EuroLab)
Warsaw, Poland
» Visit the website