ARTICLE

Qualitative LC–MS Analysis of Pesticides using Monolithic Silica Capillaries and Potential for Assay of Pesticides in Kidney
This work describes a simple and sensitive high performance liquid chromatography (HPLC) method with mass spectrometry (MS) and UV detection for the analysis of mixtures of up to 32 pesticides. The analytes from substance classes such as carbamates, organophosphates, pyrazoles, triazines or ureas were separated on C18 reversed phase monolithic silica capillary columns via a gradient elution profile and directly transferred to MS.

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COLUMNS

Column Watch
Highlights of HPLC 2013
Ronald E. Majors
A review of the trends at this year’s symposium, including discussions of column technology, sample preparation, and detector usage.

MS — The Practical Art
High Resolution Mass Spectrometry: An Ideal Premier Analytical Tool for Drug Metabolism Studies
Cen Xie, Xiaoyan Chen, and Dafang Zhong
In this installment, we introduce a way of utilizing HR-MS systems to characterize the structures of metabolites.  This results in a general workflow for metabolism studies for drug discovery and development.

LC Troubleshooting
Variable Retention Times — A Case Study
Katerina Naumoska, Alen Albreht, Irena Vovk, and John W. Dolan
How do you identify the cause of unstable retention times?

The Essentials
Secrets to Successfully Translating and Transferring HPLC Methods
There are many parameters that need to be considered when transferring HPLC methods between instruments. Knowledge of all the potential pitfalls assists with designing robust methods.

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