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Increase Efficiency for Isocratic Separations with Agilent 1290 Infinity LC-System
Siji Joseph, Agilent Technologies
The objective of this application note was to achieve lower cost for assay analysis by saving analysis time and solvent for a tramadol USP assay. This was achieved by varying column dimensions according to the currently existing or recently proposed Interim Revision Announcements (IRAs) of the United States Pharmacopeia (USP) <621> chromatography general chapters. |
Challenges in Developing an Ultra-Sensitive Bioanalytical Method for Ethinylestradiol in Human Plasma
Jessalynn P. Wheaton, Erin E. Chambers, and Kenneth J. Fountain, Waters Corporation
In this study, sensitive and selective methods for ethinylestradiol in human plasma were developed and partially validated. |
Automated Antibody Verification for Lead Discovery
Simone Greven, Birge Dübel, Heiner Apeler, Laura Main, Catherine Evans, Carsten Baessmann, Wolfgang Jabs, Bayer HealthCare and Bruker Daltonics GmbH
Within the therapeutic antibody lead discovery and optimization process the expression, purification and analytical characterization of large numbers of antibodies or antibody fragments is required. Using the software BioPharma Compass, a fully automated workflow for antibody verification has been established at Bayer Pharma Global Biologics. Based on a semi-automated sample sequence generation in combination with an automated sample acquisition, processing and report generation we were able to dramatically increase our productivity and sample throughput. |
2D Analysis for Thermoplastic Elastomers
Dr. Daniela Held, Peter Kilz, PSS Polymer Standards Service GmbH
The online combination of gradient HPLC and GPC/SEC increases peak capacity of the separations and allows to evaluate peaks which cannot be separated by either method alone.The 2D approach is the only way to determine two property distributions independently and unambiguously. A better understanding of the polymerization process and of structure - property relationships can be obtained that might help to tune products for specific applications. |
Hyaluronic Acide Analysis with Multi-Angle Light Scattering
Wyatt Technology
HAs therapeutic effectiveness depends critically on molecular weight: the higher the molecular weight, the longer its benefit. |
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Organohalide Pesticides in Water by GC–µECD with Agilent J&W DB-CLP1 and DB-CLP2 GC Columns
Ken Lynam and Doris Smith, Agilent Technologies
In this app note we demonstrate how the Agilent J&W DB-CLP1 and DB-CLP2 column pair separated organohalides from water, while enabling reliable detection at and below EU and EPA maximum contaminant levels. Calibration from 0.02 to 1.0 µg/L shows the effectiveness of the method. |
Employing a Powerful GC–MS Compound Identification Software to Accurately Identify Allergens Following Fast GC
Gareth M Roberts, ALMSCO International, ALMSCO International
Using “fast” GC–MS methods for routine analysis is appealing for rapid screening, but can increase compound co-elution for complex samples, making post-run data-analysis more difficult. This application note demonstrates how TargetView compound identification software can identify trace-level allergens in a fragrance sample, even when they are hidden under other peaks, and how conventional library searching techniques fail to achieve this. |
EPA Method 8270D for Semi-VOC Analysis
Yuri Kaplan and Ruben Garnica, PerkinElmer, Inc.
EPA Method 8270D is a common and wide ranging method used in nearly all commercial environmental laboratories. The analysis focuses on the detection of trace level semi-volatile organic compounds in extracts from solid waste matrices, soils, air sampling media, and water samples. The method lists more than 200 compounds, however a majority of laboratories target between 60 and 90 for most analyses. |
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Automated One Step SPE and Concentration of Nitrogen and Phosphorus Containing Pesticides in Water
Phillip Bassignani, FMS,Inc.
Replacing organochlorine pesticides, nitrogen and phosphorus containing pesticides are widely used both domestically and globally. Initially considered more desirable than organochlorine pesticides because of their rapid breakdown in the environment, they operate by inhibiting the enzyme acetylcholinesterase disrupting nerve function. They have been shown, particularly organophosphates, to be extremely toxic to vertebrates. |
Extraction of tricyclic antidepressants from urine using ISOLUTE SLE+
Victor Vandell and Gavin Jones, Biotage
This application note describes the fast extraction and quantitation of 10 tricyclic antidepressants in urine using ISOLUTE SLE+ supported liquid extraction columns. This simple alternative to SPE based methods provides high, reproducible analyte recoveries (>85%) and is optimized for analysis by LC-MSMS. |
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Solving Bottlenecks in Metabolite ID with the TripleTOF® Family
Hesham Ghobarah, AB SCIEX
The AB SCIEX TripleTOF® 4600 and 5600+ systems combined with MetabolitePilot™ software offer an intuitive fully integrated hardware and software solution that sets the standard in speed, sensitivity, and ease of use . In this technical note, we will provide an overview of the key benefits of TripleTOF systems technology for discovery and development metabolite ID. |
Fast Simulation of Drug Metabolism Using Electrochemistry-MS
A. Kraj, H-J. Brouwer, M. Eysberg, and J-P. Chervet, Antec
For faster, better, and superior simulation of drug metabolism, Antec has developed a dedicated electrochemistry system, called ROXY™ EC. The system is used up-front MS and allows for the generation and screening of potential metabolites, mimicking P450 enzymatic reactions in a few seconds without any biological interferences. |
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To advertise or have an application note inserted, contact:
Lee Noyes
+44 1244 629315 | Email
Lindsay Jones
+44 1244 629316 | Email
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