Transferring Methods to the Agilent 1290 Infinity LC Using Intelligent System Emulation Technology (ISET)
Angelica Gratzfeld-Huesgen, Agilent Technologies

The Intelligent System Emulation Technology (ISET) offers seamless transfer of a method for metoclopramide from a conventional LC system like the Agilent 1100 Series Quaternary LC system with higher delay volume and different mixing behavior to the Agilent 1290 Infinity LC system.

Accurate Analysis of Carbendazim in Orange Juice by LC–MS-MS
Andre Schreiber and Lauryn Bailey, AB SCIEX

Recent concerns of orange juice imports tainted with the fungicide Carbendazim, which is not approved for use in some countries, has heightened concerns about contaminants present in imported food products.  Here, we present a method for fast, accurate, and reliable analysis of Carbendazim in orange juice samples, which can be extended to screening of a wide scope of contaminants for better quality assurance of imported food products.

Fully Using Agilent High Efficiency Columns with LC/MS
Anne Mack, Applications Scientist, Agilent Technologies

Advances in Fast LC (UHPLC and HPLC) require that special considerations are made when optimizing method parameters.  In this note, various methods are evaluated to show the differences between mobile phases using MS detection with respect to ion suppression and how column particle size and data collection rates affect MS performance with consideration for sensitivity and peak capacity.

Enhanced Determination of Alcohol Biomarkers with ionBooster
Karin Wendt, Dr. Carsten Baessmann, Thomas Zey, James Hillis PhD, Bruker Daltonik GmbH, Germany. Prof. Dr. Wolfgang Weinmann, University of Bern, Switzerland, Bruker Daltonics

A new ion source, the ionBooster enables ultra low-level identification and quantification of target compounds. This application note demonstrates impressive low-level identification and quantitation of Ethyl glucuronide, a biomarker for alcohol exposure from human hair. The ionBooster combined with the maXis impact matches triple quadrupole instruments for sensitivity while offering all the benefits of accurate mass spectrometry.

Screening method for Benzodiazepines in serum
Martin Sibum, Spark Holland BV

This application note describes the method development for the analysis of 33 benzodiazepines in serum by using on-line SPE-LC-MS MS. Within a time frame of only a few days it is possible to set up this method with a recovery of more than 90% and a correlation coefficient of more than 0.99%. Manual sample preparation is no longer required, resulting in a 15 minutes runtime, including on-line SPE sample preparation.

Determination of Soy Isoflavones in Foods and Dietary Supplements by UPLC
Christopher J. Hudalla and Kenneth J. Fountain, Waters Corporation

This application note shows how the availability of the HSS Cyano phase in 5-, 3.5-, 2.5-, and 1.7-μm particle sizes facilitates scaling between different instrument platforms (HPLC and UPLC ). Method conditions can be easily scaled for different column configurations using the ACQUITY UPLC Columns Calculator. Scaling methods to utilize the XSelect HSS Cyano XP 2.5 μm Columns maximizes productivity with existing HPLC instrumentation. Additional method transfer to UPLC conditions offers the greatest savings in time and resources.