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In-Situ Calibration on Intracellular [Ca++]i, [Na+]i, and pHi
Karoly Csatorday1, Ram V. Sharma2, and Ramesh C. Bhalla2, 1Photon Technology International and 2University of Iowa, College of Medicine, HORIBA Scientific
The pH, calcium ion concentration, and sodium ion concentration were measured in cells using ratiometric dyes. Both fluorescence spectra and fluorescence image ratios can be used to calibrate such intracellular properties.
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SERS Analysis of Single Living Lymphocytes
Charlotte Eliasson, Anders Loren, Johans Engelbrektsson, Mats Josefson, Jonas Abrahamsson, and Katarina Abrahamsson, HORIBA Scientific
Surface enhanced Raman spectroscopy (SERS) can enhance the scattering cross section by several orders of magnitude, allowing species present in very low concentrations to be identified within seconds.
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Raman Analysis of Single Bacteria Cells
HORIBA Scientific with K. Christian Schuster, Ingo Reese, Eva Urlaub, J. Richard Gapes, Bernhard Lendl, Wei E. Huang, Robert I. Griffiths, Ian P. Thompson, Mark J. Bailey, and Andrew S. Whiteley
As Raman instrumentation evolves, the power of Raman in biological and medical applications is fast being realized, not least due to high information content provided and an excellent tolerance for water.
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Raman Investigation of Microorganisms on a Single Cell Level
Michaela Harz, Petra Rosch, and Jurgen Popp, HORIBA Scientific
Research shows the potential for Raman spectroscopy in combination with chemometric methods to investigate bulk samples as well as a single bacterium and yeast cell for classification on the species and strain level.
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