Application Note Alert
August 2010 Subscribe
Links to technical insights from sponsored Featured Application Notes on Chromatography Online.
Contact Editor
- Janet Kelsey
Featured Application Note:
Analysis of Polydimethyl Siloxane by GPC Viscometry with the Varian 390-LC Multi Detector Suite (MDS)
By Greg Saunders and Ben MacCreath, Varian, Inc.
This note demonstrates that PolyPore columns and the Varian 390-LC MDS multi-detection provide a powerful tool for elucidating the potential behavior of viscoelastic polymers, such as polydimethyl siloxane.
GPC
Analysis of 446 Pesticides and 1202 Transitions
By David Steiniger and Eric Phillips, Thermo Fisher Scientific, Inc.

More compounds are being added to governmental monitoring lists and screening methods for large numbers of compounds are becoming important to laboratories analyzing foods for pesticides. An instrument method was developed on the Thermo Scientific TSQ Quantum XLS triple quadrupole mass spectrometer to analyze 446 pesticides using 1202 transitions.
GPC
The Effect of NaCl Concentration on Protein Size Exclusion Chromatography
By Umbreen Ahmed, Varian, Inc.

The presence or absence of sodium chloride (NaCl) in aqueous eluents influences the elution volume of proteins by altering their hydrodynamic radius as a function of salt strength.
LC/HPLC
ALEXYS™ Clinical & Diagnostic Analyzer
By Nico Reinhoud, Hendrik-Jan Brouwer, Martin Eysberg and Joann Purkerson, Antec.

For routine analysis of catecholamines, serotonin in urine or plasma, metabolites e.g., metanephrines, VMA, HVA, 5-HIAA in urine, etc., Antec provides fast, accurate and proven applications fluidly running on its ALEXYS™ Analyzers. Click the link for a compilation of all clinical applications.

 

Metabolic profiling of tea extracts by high-resolution LC in combination with maXis UHR-TOF-MS analysis
By Aiko Barsch, Gabriela Zurek and Wiebke Lohmann, Bruker Daltonik GmbH.

An untargeted metabolic profiling experiment of different black and green tea extracts was carried out using an UHPLC and maXis ESI-TOF-MS analytical system. Processed data was submitted to principal component analysis (PCA) to identify compounds discriminating the tea varieties.  The compounds’ sum formulae were queried in public databases enabling the identification of compounds characteristic for green and black tea.

 

Automated Off-line 2-D Liquid Chromatography
By Evert-Jan Sneekes, Dionex Corporation.

This technical note describes how to resolve the maximum number of analytes possible, in proteomics research, using multidimensional separations (e.g., fractions from a cation-exchange separation are injected onto a reversed-phase column) offline.
 

Mega Dalton Protein complexes
By David Veesler, Wyatt Technology Corporation.

Recent technological advances in macromolecular crystallography led to focus on the study of more and more sophisticated biological systems, such as protein-protein complexes.

GPC
Pesticide Residue Screening in Food Samples
By Andre Schreiber, AB SCIEX.

This paper describes the use of the AB SCIEX TripleTOF™ 5600 LC/MS/MS system for targeted and non-targeted screening for pesticide residue in food samples. High resolution and accurate mass MS information was used to quantify compounds and MS/MS spectra were used to identify compounds based on mass spectral library searching.
Miscellaneous
Determination of Sulfate and Sulfamate in Topiramate
By Brian De Borba, Dionex Corporation.

This application note demonstrates an Ion Chromatography method using the IonPac AS11 column (USP L61) and a Dionex RFIC system to determine sulfate and sulfamate in topiramate that is superior to the current USP method by numerous criteria including precision, ease-of use, and simplicity.
To advertise or have an application note inserted, contact:

Lee Noyes
+44 1244393111 | Email

Lindsay Jones
+44 1244393110 | Email



You are subscribed to %%list.name%% as %%emailaddr%%. Click here to unsubscribe or edit your member profile.

To ensure delivery to your Inbox, please add %%email.list%% to your address book. If you need help doing this, click here.

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395. Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.