HPLC 2017 Topic Preview
Milos V. Novotny discusses the gaps in our biochemical understanding of glycosylated structures, and the significant challenge they represent to modern analytical methods and measurement technologies. This is the fifth in a series of articles exploring current topics in separation science that will be addressed at the HPLC 2017 conference in Prague, Czech Republic, from 18–22 June.
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A new automated cleanup system allows for fast cleanup of Dioxins and PCBs (30 minutes). Solvent consumption is limited to 110–200 mLs depending on sample lipid content. No dichloromethane is used during the cleanup steps. Sample analysis turnaround times in a few hours.
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LCGC Blog
Precise and accurate quantitative analysis based on chromatographic measurements has historically relied very heavily on careful peak integration. Seasoned analysts know that while automated algorithms exist in modern chromatography software, it is a best practice to manually check that the integration points are appropriately specified. In many cases, these practices will remain, but in some cases, where gas chromatography coupled to vacuum ultraviolet spectroscopy (GC–VUV) can be used for classification or speciation of a complex mixture, peak integration can possibly be supplanted by a new approach called time interval deconvolution (TID). read more
Application Note
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Chromatographers can maximize flexibility in their method development and analysis by adjusting selectivity without sacrificing column lifetime.  Read more
LCGC TV
In both gradient and isocratic separation modes, the most problematic part of the extra-column band broadening is behind the column. Gert Desmet from the Vrije Universiteit Brussel discusses his recent findings and offers his advice to chromatographers.
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The HILICON® iHILIC® Fusion chemistries in both column and SPE formats, address more and more polar molecule isolation challenges. Desalting with TARGA® C18, BioPureSPN™ SPE from 100% water will improve recovery of these polar molecules prior to LCMS. Try one of our  Fusion or TARGA separation approaches today! The Nest Group, Inc. - "Not Just Columns ... Answers!"
GC Connections
John Hinshaw presents an annual review of new developments in the field of gas chromatography (GC), including instrumentation, columns, and accessories, seen at Pittcon 2017 and other venues in the past year. read more
NEWS
Agilent Technologies has awarded Dwight Stoll an Agilent Thought Leader Award in support of his research applying two-dimensional liquid chromatography (2D LC) to biopharmaceutical analysis. The Agilent Technologies Thought Leader Award promotes fundamental scientific advances by contributing financial support, products, and expertise to the research of influential thought leaders in the life sciences, diagnostics, and applied chemical markets. read more
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