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21 April 2016 |
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LCGC Blog: Practical HPLC Method Development Screening
Tony Taylor considers the other important aspects of “screening” in HPLC. Discussing the importance of the mobile phase composition, gradient parameters, and flow rate when developing new HPLC methods. Offering tips and advice to ensure good efficiency, peak shape, and practice. |
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Photo Credit: Björn Kindler/Getty Images |
Analytica 2016 Preview
From 10–13 May 2016 more than 1200 exhibitors will present their latest products and developments for laboratory operations at Analytica 2016 in Munich. Susanne Grödl presents a glimpse of what can be expected at Analytica 2016. |
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HPLC 2016 Previews—Trends In Separation Science, Part 3: Reconsidering HIC for Top-Down Proteomics
Until recently, mass spectrometry (MS) was limited in the information it could supply regarding proteins larger than 40 kDa. The most recent instruments have broken through that limit, but proteins smaller than 40 kDa are still more easily detected in MS and can suppress the collection of data from larger proteins. This situation has created a demand for better separation of proteins upstream from the MS orifice. At present, though, this separation of proteins is something of a bottleneck. Methods such as reversed-phase chromatography that involve mobile phases compatible with MS are not compatible with many proteins. Alternative modes of chromatography include size-exclusion chromatography, ion-exchange chromatography, hydrophilic interaction chromatography (HILIC), and hydrophobic interaction chromatography (HIC). We decided to take another look at HIC.
This is the third in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24. |
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Flow or Velocity?
Chromatographers often use the term carrier-gas flow and velocity interchangeably when discussing column parameters. In LC, the two terms scale together, but in GC they do not: Doubling the flow does not double the velocity. This month’s “GC Connections” investigates the reasons for this non-intuitive behaviour and how it affects best practices for gas chromatographers. |
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 Photo Credit: SCIEPRO/Getty Images |
News: Quantifying Methylergonovine Using LC–MS–MS
A team of researchers from China and the USA has developed a liquid chromatography coupled to tandem mass spectrometry (LC–MS–MS) method for quantifying methylergonovine (ME), a semi-synthetic ergot alkaloid used for the treatment and prevention of postpartum haemorrhage (PPH), in human plasma. |
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Peak Purity Algorithms Using Diode Array Detectors
In an ideal chromatographic separation, all sample components would be isolated from each other and detected as fully resolved peaks. However, it is not uncommon to encounter some degree of overlap. In extreme cases, what appears to be a single peak contains in fact two or more coeluting components. This article discusses how to check the purity of such peaks in order to correctly interpret the results of the analysis. |
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