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14 April 2016 |
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Detective Work, Part 4: Chemical Problems with the Column: Chemical Attack
The fourth instalment of John W. Dolan’s Detective Work considers what happens when the mobile-phase pH is too high or too low. Exploring how the silica-based packing material in reversed-phase columns are affected when mobile phase conditions exceed the recommended limits and how to ensure you always hit the pH sweet spot. |
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HPLC 2016 Previews—Trends In Separation Science, Part 1: Why Effective HPLC Separations Are Critical for the Analysis of High-Complexity Systems
In proteomics studies, proteins are digested into hundreds of thousands of peptides, thus creating very large and complicated mixtures. Simultaneous electrospray ionization of these complex mixtures, however, results in suppression of ion formation. Therefore, it is essential to have effective chromatographic methods to separate the peptides before analysis with mass spectrometry, to relieve ion suppression and to allow the mass spectrometer sufficient time to collect tandem mass spectra of peptide ions. The challenges involved in developing such separations are great, however.
This is the first of a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24. |
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HPLC 2016 Previews—Trends In Separation Science, Part 2: New Materials for UHPLC Analysis of Monoclonal Antibody Drugs and Antibody–Drug Conjugates
Separations of intact proteins play many roles in drug discovery and development. A variety of separation techniques are used, from immunoprecipitation for study of a single protein of interest, through various types of column chromatography for detecting a handful of proteins at once, all the way to proteomics for studying hundreds to thousands of proteins. What all of these techniques and applications have in common is that the power of protein separations is limited by the fact that proteins are large, slowly diffusing, sticky molecules. This article discusses various chromatographic approaches to addressing this challenge.
This is the second in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24. |
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| Featured Application Note |
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 Photo Credit: Michael Leggero/Getty Images |
Analyzing Antarctic Ice Cores Using Capillary Ion Chromatography
Ice cores contain an abundance of information about climate and the changes it is undergoing. Brett Paull and Estrella Sanz Rodriguez from the Australian Centre for Research on Separation Science (ACROSS) at the University of Tasmania, Hobart, Australia, spoke to Kate Mosford of The Column about their work on the analysis of Antarctic ice cores and the important role of capillary ion chromatography (cap-IC) in this area of research. |
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| VICI semi prep valve |
Abalonase™ and Abalonase™+ Purified Beta-Glucuronidase |
Electronic Maintenance Indicators for Gas Filters |
The revised VICI Cheminert stream selection valves have an improved lifetime and pressure resistance (250psi liq) compared to the previous models. The C45R valves have ports for 1/4" tubing and are vailable with 4, 6 or 8 selectable positions. They are available in manual versions, or on pneumatic or electric actuators.
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UCT is excited to expand upon its enzyme hydrolysis line with the introduction of Abalonase™ and Abalonase™+, purified Beta-glucuronidase formulas. Within minutes of application, ultra-pure Abalonase™ can deconjugate the major metabolites of interest including benzodiazepines, opioids, cannabinoids (naturally occurring and synthetic) and steroids. For the deconjugation of both glucuronidated and sulfated metabolites, Abalonase™+ is enriched with 4 arylsulfatses ensuring complete hydrolysis in both human and animal based urine samples.
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Restek's electronic maintenance indicator is a monitoring device that warns you when planned maintenance for a gas filter is due. Prevent unexpected downtime by controlling your routine maintenance schedule with an electronic maintenance indicator from Restek.
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| GERSTEL MPS roboticPRO |
Wyatt |
The new MPS roboticPRO autosampler is optimized for flexibility and throughput in GC/MS and LC/MS with fast, reliable and highly accurate processing of all tasks including sample preparation and -introduction.
For more information about the MPSrobotic and other GERSTEL solutions, please contact or  visit |
The µDAWN™ is the world's first multi-angle light scattering (MALS) detector that can be coupled to any UHPLC system in order to determine absolute molecular weights and sizes of polymers, peptides, and proteins.
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