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Featured Application Note:
Phenolic Compounds in Virgin Olive Oil
Sonja Schneider, Agilent Technologies
This application note describes the analysis of hydrophilic phenolic compounds in olive oil samples, using both HPLC and UHPLC with ternary gradients on the Agilent 1260 Infinity Quaternary LC. Read More |
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Food Authenticity – Classification of Coffee Types Based on LC–MS
Verena Tellström, Sven Brand, Michael Becker, Klaus Meyer, Gabriela Zurek, and Aiko Barsch, Bruker Daltonics
The increasing global trade in food and beverages has created a commensurate demand for authentication and adulteration testing of products [1]. An example of how technology helps to determine authenticity and species purity, detect false labelling, and monitor production process control and sample similarity is the NMR-based JuiceScreener™ [2]. Combined with SGF Profiling*, the JuiceScreener™ delivers the required information from a single NMR experiment instead of multiple individual analytical steps. Read More |
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GPC Analysis of Thermoplastic Engineering Polymers (PBT, PET)
Stephen Luke, Agilent Technologies
Engineering polymers such as PBT and PET can be particularly difficult to analyze – they are generally tough and difficult to dissolve, often requiring aggressive solvents and elevated temperatures. Read More |
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SEC Analysis of Hyaluronic Acid
Stephen Luke, Agilent Technologies
Hyaluronic acid is used in exclusive pharmaceutical products, for example, in viscoelastic fluids in ophthalmological surgery and in viscosupplementary products for orthopaedic disorders. Aqueous SEC can readily determine its application and effectiveness. Read More |
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Low-molecular-weight heparins
Wyatt Technology
Size-exclusion chromatography (SEC) has been the most common way of measuring the molecular weight and molecular weight distributions of LMWHs by using the two most common detection technologies: ultraviolet (UV) coupled with refractive index (RI) detection. However, these detectors embody a relative method in order to determine molecular weights, requiring calibration standards. Read More |
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Combining technologies for the superior detection, separation, and characterization of protein aggregates
Malvern Instruments
This application note describes how size-exclusion chromatography (SEC) can be used to separate proteins and aggregates into individual populations according to their size. The addition of an extra detector, able to make measurements by both dynamic and static light scattering (DLS and SLS), allows the size, molecular weight, and oligomeric state of the populations to be measured. Read More |
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The Analysis of Chlorinated Dioxins, Difurans, and Polychlorinated Biphenyls in Edible Oils
Phillip Bassignani, FMS, Inc.
The dioxin family consists of 210 compounds, of which 17 contain the 2,3,7,8 pattern of chlorination. These 2,3,7,8-containing compounds are of extreme concern to human health because of their high level of toxicity. Approximately, 12 of the 209 polychlorinated biphenyls have also been identified as human toxins. For this reason, the US FDA and EU have established strict regulations for the monitoring of food products for human consumption, in particular edible oils. Manual extractions of oils can be a time-consuming procedure often delaying lab turnaround times. By automating the process with the PowerPrep®, food oil samples can be reliably processed with routine 24 h turnaround times. Read More |
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Analysis of Phthalates in Body Wash Using Solid-Supported Liquid-Liquid Extraction
Devon C. Zimmerman, Henry F. Rossi III, Daniel W. Keating, Jacqueline Rizzo, and Karyn M. Usher, Agilent Technologies
This application note describes the extraction and analysis of four phthalates from an infant shampoo and body wash using solid-supported liquid-liquid extraction (SLE) cartridges and an Agilent 1200 HPLC system. Read More |
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Extraction of T3, rT3, and T4 from Serum Using EVOLUTE® EXPRESS AX Plates Prior to LC–MS–MS analysis
Dr. Lee Williams, Biotage AB, Sweden
The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3, and T4 from serum. A sample of 500µL of serum was extracted using the EVOLUTE EXPRESS AX 30 mg fixed-well plate format. Read More |
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Preservation and Quantification of Hormones in Surface Water
Sonya M. Havens, James T. Schauer, and other scientists in the Environmental Chemistry and Technology Program, University of Wisconsin, Gilson
This application note describes the use of different preservatives (sodium azide, hydrochloric acid, and sulphuric acid) to inhibit the degradation of hormones in samples of surface water runoff from cattle manure-amended fields during storage at 4 °C. The hormones and hormone metabolites were extracted from surface water using the automated Gilson GX-271 ASPEC™ System. Read More |
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Determination of Norfentanyl / Fentanyl in Urine Samples Using Solid-Phase Extraction and GC–MS
Jeff Hackett, UCT
Fentanyl is a potent, synthetic opioid analgesic. It has been used to treat breakthrough pain. Typically, analysis for fentanyl by GC–MS has focused on the extraction and quantification of the parent compound, not the primary metabolite norfentanyl. Additionally, previous analysis of the primary metabolite has involved derivatization of the compound. In this methodology, both compounds were extracted from urine using mixed mode SPE followed by GC–MS analysis. Read More |
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