High-Resolution Peptide Mapping Separations with MS-Friendly Mobile Phases and Charge-Surface-Modified C18
Waters Corporation
This work demonstrates that a novel charged-surface C18 (CSH C18) stationary phase alleviates some of the challenges associated with reverse-phase peptide separations. CSH C18 columns improve loadability, increase peak capacities, and offer unique selectivity compared to conventional C18 columns. Performance is significantly less dependent on TFA-ion pairing, making it ideal for MS applications where high sensitivity is desired. Read More |
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Increasing Peak Capacity in Reversed-Phase Peptide Separations with Charged Surface Hybrid (CSH) C18 Columns
Waters Corporation
In this study, we evaluate charged surface hybrid (CSH) C18 column chemistry for peptide separations, expecting similar improvements in chromatographic performance since most peptides under commonly employed acidic conditions contain a positive charge. This work with peptides demonstrates that this novel stationary phase exhibits greater peak capacity, unique selectivity, and less dependence on MS signal suppression, strong ion pairing agents in peptide analysis. Read More |
Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases
Waters Corporation
In this study, the use of CSH130 C18 Column with FA mobile phases is further investigated with the analysis of more demanding separations. LC–MS of an enolase tryptic digest is compared among a CSH130 C18, BEH130 C18, and a superficially porous C18 column. In addition, the applicability of these columns for separations of polypeptides up to 12 kDa is evaluated. Read More |
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High Mass Loading of Peptides with Hybrid Particle C18 Columns and Acetic Acid Mobile Phases
Waters Corporation
This study investigates the use of both BEH C18 and CSH™ C18 Columns for preparative peptide separations. The presented data demonstrate that both BEH C18 and CSH C18 Columns can yield narrower target peaks at high mass loads with optimized acetic acid mobile phases than those containing 0.1% TFA. Read More |
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Future-Proofing the Biopharmaceutical QC Laboratory: Integrating AutoBlend Technology to Improve Peptide Mapping
Waters Corporation
This application note demonstrates the ability of Waters Auto•Blend Technology to control the trifluoroacetic acid (TFA) component of the mobile phase during routine peptide mapping analyses, thereby producing chromatograms of equivalent performance to HPLC-acquired chromatograms with conventionally modified mobile phases. Read More |
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Charged Surface Hybrid C18 for High Peak Capacity nanoLC Peptide Separations
Waters Corporation
We demonstrate the unique ability of charged surface hybrid (CSH™ Technology) C18 to produce high peak capacity peptide separations at high mass loads in nanoLC chromatography. Read More |
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Optimizing Peak Capacity in Nanoscale Trap-Elute Peptide Separations with Differential Column Heating
Waters Corporation
We discuss the concepts to be considered to achieve high peak capacity nanoscale trap-elute peptide separations. A 15 K psi capable, ACQUITY UPLC M-Class System is used to demonstrate high peak capacity trap-elute peptide separations with a charge surface hybrid (CSH) C18 analytical column. Read More |
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An Introduction to the Capabilities of Microscale 2D-RP-RP Peptide Chromatography with an ACQUITY UPLC M-Class System
Waters Corporation
This technology brief demonstrates that 2D-RP-RP chromatography operated at ≥10 K psi with an ACQUITY UPLC M-Class and an ACQUITY UPLC M-Class 300 µm analytical column can be a robust solution for obtaining high peak capacity. It will be shown that such a system affords high resolving power and outstanding chromatographic reproducibility and performance with extended use. Read More |
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