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Screening Environmental Samples for a Diverse Range of Compounds with Accurate Mass LC-MS and an Integrated Scientific Information System
The presence of an increasingly complex array of pharmaceuticals and personal care products (PPCPs) in water bodies throughout the world is placing a greater demand on techniques used to screen for these compounds. In combination with UNIFI, an integrated scientific information system, it is now possible to screen for the presence of PPCPs, their fragments, adducts, and potential metabolites in a routine laboratory environment. This application note describes the use of Waters Screening Platform Solution in combination with the UNIFI Toxicology library to screen a local well water sample for the presence of a large number (>1000) of PPCPs, pesticides, and drugs of abuse. Read More |
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A Label-Free, Multi-Omic Study to Qualitatively and Quantitatively Characterize the Effects of a Glucosylceramide Inhibitor on Obesity
A label-free, multi-omics approach provided qualitative and quantitative information in a single experiment to help characterize and investigate the effects of a glucosylceramide inhibitor for treatment of obesity. Progenesis QI and Progenesis QI for proteomics software enabled a seamless workflow, from LC–MS data processing and database searching to pathway interrogation, implicating carbohydrate metabolism, and molecular transport networks in the compound's mechanism of action. Read More |
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Using the Elucidation Tool in UNIFI Scientific Information System to Identify Unknown Compounds in Natural Products
To identify components from a sample that either were not matched with scientific libraries within the UNIFI Scientific Information System, or were matched but were suspected to be false positive, the Elucidation Tool within the UNIFI can be used. An example is used to demonstrate the process using the Natural Product Application Solution with UNIFI. Read More |
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A Reproducible Method for the Quantification of Pioglitazone and Metabolites in Human Plasma Using the ACQUITY UPLC H-Class System and Xevo TQD MS with UNIFI
A high sensitivity method was developed for the analysis of pioglitazone and its two active metabolites, keto pioglitazone and hydroxy pioglitazone, in human plasma. The extremely low carryover exhibited by the ACQUITY UPLC H-Class System allows the full sensitivity of the Xevo TQD Mass Spectrometer to be utilized. Read More |
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21 CFR Part 11 Readiness of Empower Chromatography Software
Meeting 21 CFR Part 11 compliance remains challenging; however, e-record regulations will eventually be viewed as a significant driver to move companies from a paper-records environment to a more efficient electronic-records environment. Although it is understood that merely purchasing a chromatography data software package that incorporates Part 11 technical controls does not make one fully compliant, technical controls should be inherent in any system used in a regulated environment. A suite of technical controls for 21 CFR Part 11 compliance are built into Empower 3 Software to simplify administration and allow laboratories to meet the U.S. and European regulations. Read More |
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Addressing Three Problems with Information Search in Scientific Organizations
Access to information is central to the success of science-driven organizations. These organizations need an informatics solution that provides timely, regular, accurate, and useful search results to deliver benefits across multiple layers of the organization. A new generation of searching solution is required for science-driven organizations: one that extends across internal information repositories, handles science objects, and can index unstructured data. Read More |
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Is it Time to Replace Your Chromatography Data System?
Learn about the three main drivers for considering a new chromatography data system (CDS) and how your lab can benefit. Obtain guidance in making the decision to replace an existing CDS and get practical "factors to consider" that address common concerns. Read More |
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Increasing Efficiency of Method Validation for Metoclopramide HCl and Related Substances with Empower 3 MVM Software
The validation process of an analytical method is a complex and demanding activity consisting of many time-consuming steps. Some of these steps include creation of validation protocols, experimental work, reviewing and processing data, performing calculations, approving, and final reporting. Since some of these steps are prone to errors, a well-organized plan is essential for successful validation of an analytical method and to ensure that the appropriate regulations and guidelines are being followed. Read More |
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Combining Mass and UV Spectral Data with Empower 3 Software to Streamline Peak Tracking and Coelution Detection
Methods development using a single detection technique can be challenging. UV spectral data can assist in peak purity and identification however it is difficult to perform peak tracking in the presence of coelutions. Mass spectral data can be used to match chromatographic peaks to items on a short list of compounds, but the presence of isobaric compounds can require additional information: complete separation is typically required and not only allows for immediate identification based on the UV but also more reliable quantification. Typically, identifying these phenomena requires further analyses. For simplified methods development, a chromatographic system using both UV and mass detection and a streamlined software platform can be combined to evaluate peak purity and assist in the identification of coelutions in a single run. Read More |
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