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Comparative Analysis of Cooking Oils Using a Solid Core HPLC Column
Mark Tracy and Derek Hillbeck, Thermo Fisher Scientific
The use of Thermo Scientific™ Accucore™ C30 columns for the characterization of different cooking oils is demonstrated. The C30 phase offers greater selectivity and resolution of minor components when compared to the C18 phase. |
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Determination of Catechins and Phenolic Acids in Red Wine by Solid Phase Extraction and HPLC
Monica Dolci, Thermo Fisher Scientific
This application note demonstrates a simple and rapid HPLC method for the analysis of nine catechins and phenolic acids in red wine. Extraction of these polar analytes was achieved on Thermo Scientific™ HyperSep™ Retain PEP material. The polyphenols in the extracts were quantified by a matrix-standard calibration, with extracts from a wine sample spiked with increasing amounts of analytes. Determination of the polyphenols was performed by HPLC, using a Thermo Scientific™ Accucore™ PFP HPLC column under gradient mobile phase conditions. |
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Analysis of Cephalosporins on a Polar Endcapped C18 3 µm Particle Column
Valeria Barattini, Thermo Fisher Scientific
The analysis of species containing polar groups using reversed phase HPLC can be challenging. The polar groups present on the analytes can result in secondary interactions with silanols on the silica surface. Furthermore, the overall polarity of the molecule sometimes calls for the use of mobile phases with very high aqueous content to ensure the analyte solubility, retention, and elution, at the expense of stationary phase stability. In this application note we demonstrate the analysis of a mix of four analytes from the cephalosporin antibiotics family on a Thermo Scientific™ Syncronis™ aQ 3 µm particle column. The analysis yields excellent peak shape and resolution in short run times, as well as outstanding reproducibility. |
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