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Featured Application Note:
Generic RP-HILIC–UHPLC Method for APIs and Impurities
Frank Steiner, Michael Heidorn, and Markus M. Martin, Thermo Fisher Scientific
This generic method addresses the need to make changes during the drug development life cycle and is applicable to each new drug formulation. By using gradient RPLC in the first stage and HILIC in the second stage, both hydrophilic and hydrophobic compounds are separated in a single, short run. Read More |
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Novel Approaches for Small Molecule Identification
Gabriela Zurek, and Aiko Barsch, Bruker Daltonics, Inc.
In plant metabolomics, confirmation of the structure of known compounds and structural elucidation of unknown compounds represents a bottleneck in the analysis of secondary metabolites. In this application note, we present novel tools that facilitate the identification process of natural products when mass spectral libraries are not available and sample amounts are limited. This information can subsequently be used to build well-characterized MS–MS libraries, enabling quick dereplication of known compounds. Read More |
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Automated Solid Phase Extraction (SPE) and Concentration of Organochlorine Pesticides in Water
Philip Bassignani, FMS, Inc.
Organochlorine pesticides are man-made organic chemicals with a history of wide spread use throughout the world. Tending to be very persistent in the environment, they have found their way into sediments and drinking water supplies posing serious health risks. The automated solid phase extraction and concentration method described allows for rapid, reproducible extractions using minimal solvent volume that produces consistent results. Read More |
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Fast Extraction of VMA, 5-HIAA, and HVA from Synthetic Urine using EVOLUTE EXPRESS AX
Victor Vandell, and Frank Kero, Biotage AB, Sweden
This application note describes the extraction of three clinically important acidic catecholamine metabolites from urine with analysis by LC–MS–MS. The use of EVOLUTE EXPRESS AX plates using the fast load-wash-elute procedure eliminates the need for traditional conditioning and equilibration steps. Read More |
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The First RI Detector for UHPLC Detection
Dan Some, Wyatt Technology
Recognizing the need for an on-line UHPLC dRI detector, Wyatt Technology developed the first such instrument: The Optilab UT-rEX launched in February 2013. The UT-rEX meets the needs of UHPLC thanks to greatly reduced band broadening, maintaining the narrow peak widths characteristic of UHPLC, as well as a higher data rate to accommodate the combination of narrow peaks and high flow rate. Read More |
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Measurements of Absolute Molecular Weight of Synthetic Polymers using the Viscotek SEC-MALS 20
Malvern Instruments
In this application note, the molecular weight, molecular weight distribution, and size of two common polymers are measured using the new Viscotek SEC-MALS 20. The results from SEC-MALS are compared with those from conventional calibration and the differences are discussed. Read More |
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Thermal Treatment Analysis of Virgin Olive Oils
Sonja Schneider, Agilent Technologies
3,5-stigmastadienes were analysed in seven olive oil samples using the Agilent 1220 Infinity Mobile LC Solution to differentiate virgin from refined or other thermally-treated olive oil. Read More |
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Fast Separation of Food Preservatives Using an Agilent Poroshell 120 EC-C18 Column
Rongjie Fu, Agilent Technologies
An UHPLC method was developed for the simultaneous determination of the nine preservatives most often used in food and beverages. An Agilent Poroshell 120 EC-C18 column was used for the separation, with a gradient method and acetate ammonium buffer:acetonitrile mobile phase. This method gave a rapid separation of the preservatives in 9 min. It is suitable for a variety of food and beverage samples and was applied here to preservatives in a cake sample. Read More |
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