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Featured Application Note:
Separation of 2AB Labeled N-Glycans from Bovine Fetuin on a Novel Mixed-Mode Stationary Phase
Udayanath Aich, Xiaodong Liu, Srinivasa Rao, Chris Pohl, Thermo Fisher Scientific
In this note we demonstrate unique selectivity and excellent resolution for glycans released from fetuin. This is shown with the analysis of fluorescently labeled N-glycans released from proteins (bovine fetuin) and separated by hydrophilic interaction liquid chromatography (HILIC) / weak anion exchange mixed-mode chromatography. The separation is carried out with a 1.9 µm Thermo Scientific™ GlycanPac™ AXH-1 column using fluorescence detection. The method exhibits excellent separation based on charge, size, and polarity. This phase also provides a unique tool for quantifying glycans based on charge. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans. Read More |
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SEC-Light Scattering for Antibody Drug Conjugate Characterization
Wyatt Technology
Among the factors critical to effective ADC design is the drug antibody ratio (DAR). The DAR describes the degree of drug addition, which directly impacts both potency and potential toxicity of the therapeutic, and can have significant effects on properties such as stability and aggregation. Determination of DAR is, therefore, of critical importance in the development of novel ADC therapeutics. We present here a method for DAR determination based on SEC-MALS in conjunction with UV absorption and differential refractive index detection. Read More |
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Effect of Gamma-Ray Sterilization on High Molecular Weight PMMA
Malvern Instruments Ltd.
A sample of PMMA for medical applications was analyzed, before and after sterilization, by size exclusion chromatography with triple detection (SEC-TD) in order to characterize any changes the irradiation brought about. By measuring intrinsic viscosity, cleavage of the polymer at the branching points was identified as the cause of the reduction in molecular weight. This application note provides important information about the process of sterilizing PMMA, as well as the benefits of multi-detector characterization. Read More |
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EPA Method 8270 SVOC Analysis Using the 5977A GC/MSD
Dale R. Walker, Agilent Technologies
The US Environmental Protection Agency Method 8270 analysis utilizes GC–MS to analyze solid, liquid, and gaseous samples for semivolatile organic compounds. Method 8270 challenges include the simultaneous measurement of acid, base, and neutral compounds over a wide concentration range. Read More |
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Time-of-Flight Mass Spectrometry Applied to the Identification and Quantitation of Trace-Level Compounds in Beer
Steve Smith and David Barden, Markes and ALMSCO International
This application note shows that BenchTOF-dx is a powerful detector for the trace-level flavor compounds present in the complex profiles resulting from the sorptive extraction of beer. The generation of linear responses at sub-ppb levels is a particular feature of the work, while the ability of BenchTOF-dx to produce "classical" spectra matching those in commercial libraries greatly simplifies automated compound identification. Read More |
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Complex Mixture Analysis Using Hydrogen as a Carrier Gas
Ed Connor, Peak Scientific
The following technical brief aims to demonstrate the performance of GC–MS using hydrogen as a carrier gas. Here we compare the performance of hydrogen and helium for the analysis of a complex mixture. The results show that by using hydrogen, chromatographers can achieve superior results to helium with faster run times and taller, sharper peaks. Read More |
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Dioxins, PCBs, and PBDEs in Human Serum Using Automated Pressurized Liquid Extraction, Multi-Column Cleanup, and Concentration
Philip Bassignani, FMS, Inc.
Because of its complexity, human serum is one of the most challenging sample matrices encountered. Organic contaminants often exist at low lipid concentrations (~600 mg/dl) that require extremely low detection levels and ultra clean blanks. The manual sample preparation process for human serum consists of multiple, time-consuming steps that are messy, difficult to reproduce, and yield inaccurate results. By combining the analysis of multiple analytes into a single extraction and concentration method using the pressurized liquid extraction (PLE) system, the PowerPrep system to fractionate analyte classes, and the SuperVap Concentrator, the sample preparation process can be streamlined into a rapid, reproducible method. Read More |
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Extraction of 45 multiclass drugs of abuse using ISOLUTE SLE+ with LC-MS/MS analysis
Dr. Lee Williams, Biotage AB, Sweden
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of 45 drugs of abuse (including amphetamines, opioids, benzodiazeplines, cocaine and metabolites) from hydrolyzed urine prior to LC-MS/MS analysis. Sub ng/mL levels were achieved for all analytes using 100 µL of urine on the fixed well plate format. Read More |
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Solid-Phase Extraction of Pesticides in Water using Graphitized Carbon Black (GCB)
Xiaoyan Wang, UCT, Inc.
Graphitized carbon black is a reverse phase and anion exchange sorbent. GCB retains non-polar compounds (e.g. organochlorine pesticides) and some polar compounds (e.g. surfactants), which are difficult to retain by reverse phase sorbents. This SPE method uses UCT's treated GCB for the determination of pesticides in water providing excellent recovery. Read More |
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Simultaneous Quantitative and Qualitative Drug Metabolism Investigations
Don Richards, Bruker Daltonics
The ability to simultaneously collect quantitative and qualitative information from a DMPK-analysis has the potential to significantly increase productivity in pharmaceutical drug discovery and development. This work describes an investigation using the latest quadrupole-time-of-flight mass spectrometer to obtain clearance data, metabolite identification, structure elucidation, and metabolite profiles from Cytochrome P450 microsomal incubations in a single sample set. Read More |
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Illegal dyes in spices
H. Kandler et. al, CAMAG
Illegal dyes in spices In the past years illegal azo dyes have been detected in food in several European countries. These red and orange dyes are classified as carcinogens and were used to artificially enhance the natural color of spices. This validated high performance thin layer chromatography (HPTLC) method is suitable for the screening of chili, paprika, curry powder and spice mixtures for the presence of Sudan I, II, III, IV, Sudan Red B, Sudan Red 7B, Sudan Red G, Para Red, FD&C Orange 2, Butter Yellow, Citrus Red 2, Toluidine Red, and Disperse Orange 11. Read More |
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