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April 2013 Clinical Research Biopharmaceutical Analysis Pharmaceutical Analysis Food Authenticity Food Safety Environmental Analysis Comprehensive LC-MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine Peter J.W. Stone, Agilent Technologies, Inc. The Agilent 6420 Triple Quadrupole LC-MS system is used to detect a wide variety of analgesic and pain management analytes in urine samples with minimal sample preparation while providing sufficient sensitivity, quantitative linearity, and accuracy. Read More Rapid Analysis of 25-OH Vitamin D in Serum Using an Agilent Triple Quadrupole LC-MS System with Automated Online Sample Cleanup Rory Doyle, Agilent Technologies A rapid and reliable LC-MS research method for accurate, sensitive, and reproducible quantification of 25-OH vitamin D in serum was developed. Automated online cleanup further minimizes matrix interference and ion suppression. Read More Sensitive Detection of Three Forms of Thyroid Hormone in Human Serum Rory Doyle, Agilent Technologies A rapid method for the quantification of free and total thyroid hormones in serum was described using an Agilent LC coupled to a Triple Quadrupole. Simple filtration procedure for free concentrations and a liquid-liquid extraction for total concentrations were applied. Read More Detection of Opioids and Opiates in Dried Blood Spots Using the Agilent Automated Card Extraction LC-MS System Kevin McCann, Agilent Technologies Agilent's Automated Card Extraction LC-MS system facilitates rapid analysis of DBS cards, reducing hands-on time while maintaining sensitivity, accuracy, and reproducibility. Read More   Analysis of Polyethylene Glycol (PEG) and a Mono and Di-PEGylated Therapeutic Protein Using HPLC and Q-TOF Mass Spectrometry Ravindra Gudihal, PhD, Agilent Technologies A method using LC-MS is described for improving the analysis PEGylated peptides and proteins using the post-column addition of a charge stripping agent to obtain simpler mass spectra for detailed structural characterization. Read More   Purity Assessment of Drug Discovery Compound Libraries Using an Agilent Single Quadrupole LC-MS System Coupled to Diode Array and Evaporative Light Scattering Detectors N.S. Kameswara Rao, PhD, GVK Biosciences Fast and automated purity assessment of a 30 compound library using an Agilent 6130 single quadrupole LC-MS system coupled to both a UV and an ELSD detector, enables the detection of impurity peaks that may be missed by using only one mode of detection. Read More Pharmaceutical Impurity Identification and Profiling Using Agilent Q-TOF LC-MS Combined with Advanced MassHunter Data Processing Software Siji Joseph, PhD, Agilent Technologies This app note describes an efficient software-assisted workflow for impurity identification, profiling of active pharmaceutical ingredients and their structural elucidation using MS and MS-MS on a high resolution accurate mass Q-TOF LC-MS system. Read More   Metabolomic Profiling of Wines using LC-QTOF MS and MassHunter Data Mining and Statistical Tools Lucas Vaclavik, PhD, Institute of Chemical Technology A non-targeted metabolomic analysis approach to classify wine variety was developed. Molecular feature extraction, data filtering, and statistical analysis identified 26 compounds used to build a prediction model with 95.6% overall accuracy in discriminating certain red wine varieties. Read More   Determination of PFOS and PFOA in Food Matrix of Animal Origin Using UHPLC Hyphenated Triple Quadrupole Tandem Mass Spectrometry Dunming Xu, Xiamen Entry-Exit Inspection and Quarantine Technical Center An ultrasensitive method has been developed for simultaneous determination of perfluorooctane sulfonates (PFOS) and perfluorooctanoic acid (PFOA) in food using Agilent UHPLC coupled to a 6460 Triple Quadrupole mass spectrometer. High sensitivity, high recovery, and good selectivity were demonstrated. Read More   Sensitive LC-MS Quantitation of Trace Organic Contaminants in Water with Online SPE Enrichment Tarun Anumol, Ph.D., University of Arizona An online SPE method has been used for the rapid, sensitive, reproducible, and robust analysis of trace organic compounds in water. Online SPE accommodates large sample volumes and eliminates tedious extraction procedures. Read More   Go to Application Note Library Subscribe to the Application Note Alert LCGC Print Edition LCGC Digital Edition