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April 18, 2013
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The LCGC Blog

The LCGC Blog: Things You Should Know About Your HPLC Column, Part I - Pore Sizes and Particle Diameters
The physical characteristics of silica-based HPLC columns can affect the performance of the separation almost as much as the bonded phase. This month's technical tip re-visits some of the lesser known or remembered facts relating to silica particles used for chromatography.
Succeed from Sample Prep to Preparation
Agilent offers a complete portfolio of Fast LC columns—conventional HPLC or UHPLC and biocolumns in reversed phase, size exclusion, ion exchange and affinity—plus the sample prep chemistries you need to perfect your analysis. Bring your separations together with Agilent LC tools and resources at
Featured News

First Institution in India Recognised by Waters Centers of Innovation Program
Waters (Milford, Massachusetts) has announced the selection of the St John's Research Institute (SJRI)(Bangalore, India) - known for its proteomics research - for it's Centers of Innovation program. St John's is the first research institution in India to join the program.
Offering the industry’s broadest selection of standard and custom fused silica capillary tubing, Polymicro™ products provide unmatched design flexibility and the tightest tolerances available. Ideal for a variety of scientific applications, we now offer a range of 1/32” OD capillary tubing. For products you can trust, turn to Polymicro from Molex. Learn more

World's Biggest Allergy Study
The University of Manchester (Manchester, UK) has announced the start of the world's biggest allergy study. Eurofins (Brussels, Belgium) is one of the main industry laboratory participants in the four-year study sponsored by the European Commission.
Hamilton HPLC Column Compound Application Index - Online!
Three clicks and you have access to the growing list of almost 1400 compounds that can be separated on Hamilton polymer-based HPLC columns.
•Compounds listed alphabetically
•Keyword search makes it easy to get chromatograms and separation conditions
•Receive helpful literature references
Try the HPLC Column Application Index today!
Market Profile: UHPLC Versus Standard HPLC
Ultrahigh-pressure liquid chromatography (UHPLC) systems are designed to take full advantage of sub-2-µm particles, which yield higher resolution and increased sensitivity.
Download LCGC's New E-Book- Innovations in Food Analysis
Whether you are conducting food safety testing, nutritional research, or product development, you need the latest methods. This new special issue is here to help by bringing together vital information on methods for food analysis. Learn more

LC Troubleshooting
Need Help With LC Troubleshooting?

A free interactive LC Troubleshooter is now available at CHROMacademy, sponsored by Thermo Scientific. In 3 simple steps you will overcome your instrument, separation and quantitation issues. It's completely free. More.

5 Years Free Access to CHROMacademy
To show our commitment to learning, we have decided to offer all university students and staff free access to CHROMacademy.  You are the future leaders and innovators of the scientific community, and by offering free access to CHROMacademy we hope to help you realize your potential.  This has been made possible by Agilent Technologies- the world’s premiere measurement company. Learn more
Featured Product
Kinetex core-shell technology
Shockingly better performance than your current UHPLC/ HPLC/ PREP LC column!
Immediately improve resolution, productivity, and sensitivity of your current sub-2 µm, 3µm and 5µm methods with Kinetex core-shell technology. Learn more
First Refractive Index Detector for UHPLC
Wyatt Technology announces the immediate availability of the Optilab UT-rEX (UHPLC refractometer with EXtended range), the first RI detector specifically designed for use with UHPLC systems using columns packed with small beads. Using a combination of miniaturized components and cutting-edge semiconductor photodiode technology as well as proprietary computer algorithms, the UT-rEX presents a host of instrument firsts. Read more
Application Note
Validation of PAHs in Soil and Sediment Samples Using Pressurized Liquid Extraction, Cleanup, and Concentration
FMS, Inc.

PAHs are a group of organic compounds consisting of two or more benzene rings and are often the byproduct of petroleum combustion. Due to their carcinogenic characteristics at relatively low concentrations, they are of particular environmental concern. Seven PAHs have been classified by the US EPA as probable human carcinogens and their toxic characteristics and persistent nature place them among the most extensively monitored organic contaminants. The following procedure details the use of pressurized liquid extraction (PLE) in conjunction with automated silica gel cleanup (PowerPrep) to deliver a robust, efficient sample prep process for soils and sediments designated for PAH analysis. Outlined, are an initial precision and recovery study (IPR), a matrix detection level study (MDL), and a matrix validation of a NIST reference material.
Read More.
New Webcasts
Editors' Series: Industrial Problem Solving Using Ion Chromatography
Wednesday, April 24, 2013
8:00 AM PDT; 11:00 AM EDT.
This web seminar will highlight the importance of IC for industrial problem solving and provide insights into some practical approaches used. Newer applications for IC in the area of total heteroatom determination using combustion-IC will also be introduced.
Advancing Environmental Methods with Application-Specific Stationary Phases
Wednesday, April 25, 2013
11:00 AM EDT.
This webinar will discuss the strategies laboratories can use to increase productivity by leveraging environmental-specific chemistries and technologies.
Editors' Series: Faster Gas Chromatography in Practice
Wednesday, May 22, 2013
11:00 AM EDT.
Want faster GC separations? Tune in to learn a convenient method for selecting the most appropriate route for speeding up specific GC applications.
Editors’ Series: A Generic HPLC-UV Platform Method for Cleaning Verification
Wednesday, June 26, 2013
11:00 AM EDT.
Tune in to learn about the development and qualification of a simple, 10-min generic HPLC-UV gradient method for quantitative assays used in cleaning verification in a pharmaceutical environment.
Upcoming Events

23-25 April 2013
Interphex 2013
New York, NY
» Visit the website
24 April 2013
Comprehensive Capillary GC
Chicago, IL
» Visit the website
26 April 2013
Comprehensive Capillary GC
Indiapolis, IN
» Visit the website
28 April–1 May 2013
AOCS (American Oil Chemists' Society) - Oil
Montréal, Canada
» Visit the website
29 April 2013
GC-MS Fundamentals
Orlando, FL
» Visit the website
28 April-1 May 2013
ISBT BevTech 2013
Fort Lauderdale, FL
» Visit the website
30 April-1 May 2013
Environmental Trade Fair and Conference
Austin, TX
» Visit the website
1 May 2013
GC-MS Fundamentals
Atlanta, GA
» Visit the website
12-16 May 2013
37th International Symposium on Capillary Chromatography and 10th GCxGC Symposium
Palm Springs, CA
» Visit the website
15-17 May 2013
The 44th ACS Central Regional Meeting
Mount Pleasant, MI
» Visit the website
19-24 May 2013
FOA 2013, International Symposium on Fundamentals of Adsorption
Baltimore, MD
» Visit the website
20-24 May 2013
Bejing, China
» Visit the website
10-12 July 2013
7th International Conference on Packed Column SFC
Boston, MA
» Visit the website