The LCGC Blog
I hear the words "struggling for sensitivity" so often when speaking to folks using LC–MS for bioanalysis, environmental analysis, metabolomics, proteomics, and a host of other applications where target analytes are present at low concentrations in complex matrices. We spend fortunes on MS/MS instruments to increase specificity of detection in order to improve sensitivity. Some of us go to great lengths to optimize sample extraction and HPLC conditions in order to minimize matrix suppression effects and improve specificity and hence sensitivity. Read more
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Antibody–drug conjugates (ADCs) are very important cytotoxic drugs. Alain Beck from the Center of Immunology Pierre Fabre discusses the development of a two-dimensional liquid chromatography (2D-LC) method for quantifying free drug species in ADC samples, and the importance of quantifying free drug species. Read more
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Featured Articles
To resolve and quantitate monoclonal antibodies and antibody–drug conjugates, analytical scientists need various chromatographic modes, such as size-exclusion, ion-exchange, hydrophobic interaction, and hydrophilic interaction chromatography. Here, we provide advice for making the most of each mode. Read more
Dwight Stoll, who will take the reins of "LC Troubleshooting" next month, spoke with John Dolan to get some insight on the current state of chromatography with John Dolan to get some insight on the current state of chromatography training, future troubleshooting problems, John's toughest troubleshooting challenge, and the most common chromatographic mistakes. Read more
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New HIC-MS chemistries for Top Down proteomics of intact proteins. Conventional HIC materials, may not adequately retain proteins using ammonium acetate buffers. RPC may be too hydrophobic to not denature or precipitate proteins. The new PolyPENTYL A™ chemistry from PolyLC™ addresses this. Characterize  large protein variants by HIC-MS on your MS today! The Nest Group, Inc. - “Not Just Columns ... Answers!”
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Comparability assessments always have two basic questions: Is the new material the same or is it different? If it's different, what do the differences mean for patients? Reed Harris from Genentech discusses if the approach to comparability assessments needs to vary depending on whether one is talking about a commercial product or one still in development. Read more
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Featured Articles, Continued
The disinfectants commonly used to treat public drinking water can react with naturally occurring organic and inorganic matter in the source water to form disinfection byproducts such as haloacetic acids. Here, we describe the use of two-dimensional matrix-elimination ion chromatography (MEIC) for haloacetic acid analysis. This method minimizes the impact of matrix ions. Read more
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Application Notes
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The below Clean Screen® XCEL I method can be utilized for both universal screening and confirmatory purposes when extracting various drugs from oral fluid prior to analysis by LC-MS/MS. By evaporating to completion, final extracts are purified and highly concentrated to achieve required levels of detection/quantitation.  read more |
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A sample prep method using PPT followed by Agilent Captiva Enhanced Matrix Removal—Lipid cleanup for the quantitative determination of nine drug compounds.  read more |
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This paper illustrates "best practices" use of TLC prior to flash chromatography with simple examples, including definitions of RF, CV, and use of gradient chromatography.  read more |
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