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December 2015 SAMPLE PREP GPC HPLC APP NOTES   Analyzing Infant Formula for Dioxins and Furans Using Pressurized Liquid Extraction and Automated Sample Clean Up Because of important public health considerations for children, infant formula has been tested for Persistent Organic Pollutants (POPs) such as polychlorinated dibenzo-p-dioxins (PCDDs) and furans (PCDFs). Typically, concentrations of these analytes found are lower than in human breast milk but continuous monitoring is important. This requires well equipped laboratories capable of trace level analysis work, as often PCDD/Fs amounts are at the picogram or femtogram level. Automation reduces time, labor solvent and error. Download the app note Absolute Molar Mass in UHPLC via the µDAWN and UT-rEX Ultrahigh-performance liquid chromatography (UHPLC) enables fast, efficient separation of biomolecular samples. Compared to standard SEC, UHPLC�SEC provides improved resolution, higher throughput, less solvent, and smaller sample consumption for analysis of precious biological samples. Download the app note Automated Scouting of Stationary and Mobile Phases This technical overview demonstrates the use of the Agilent 1290 Infinity II Method Development Solution for automated scouting of stationary and mobile phases. Download the app note Purification of a Monoclonal Antibody with TOYOPEARL® AF-rProtein A HC-650F at Various Bed Heights A variety of column dimensions in terms of internal diameter and length are used by scientists when developing a protein A purification step for monoclonal antibody capture. Typical bed heights for protein A columns used in a commercial manufacturing process are in the range of 15 to 20 cm in order to optimize throughput, yield, and recovery to make the production as efficient and cost effective as possible. Here, we show that altering column dimensions to favor a shorter, wider column will have no effect on resin performance when compared with a longer, narrower column of the same total volume. Download the app note Determination of Cylindrospermopsin and Anatoxin-a in Drinking Water by Aqueous Direct Injection and LC–MS-MS This application note describes a direct aqueous injection and liquid chromatography tandem mass spectrometry (DAI–LC–MS-MS) method for the determination of cylindrospermopsin (hepatotoxin) and anatoxin-a (neurotoxin) in drinking water under EPA Method 545. Download the app note Sample Preparation Using Hamilton Microlab 600 Environmental sample preparation using the Microlab 600 is accurate and fast. The Microlab 600 has small dilution volumes, an inert fluid path, and is a rapid, accurate technique. Download the app note   Go to Application Note Library Subscribe to the Application Note Alert LCGC Print Edition LCGC Digital Edition