Featured Application Note:
SPE Cartridges Simplify Organic Extractions
Jennifer Peterson, David Knowles, Richard Carlson, and Bruce Richter, Thermo Fisher Scientific
Learn how to quantify eight benzodiazepines were quantified with LLOQs at the pg/mL level and with linear dynamic ranges of 3 to 4 orders of magnitude using the Thermo Scientific Q Exactive high performance benchtop mass spectrometer.
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Extraction of Antioxidants from High Density Polyethylene (HDPE) by Pressurized Liquid Extraction (PLE)
Phil Bassignani, FMS, Inc.
Plastics and other polymers are used in a wide spectrum of applications from industrial usage to packaging, including the packaging of food and pharmaceutical products. To enhance functionality, chemical additives are often added to polymers. This application note demonstrates the use of pressurized liquid extraction using the FMS PLE system to extract polymer additives for quatitative analysis by HPLC. |
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Determination of Polycyclic Aromatic Hydrocarbons(PAHs) in Seafood Using GC–MS
Don Shelly and Craig A Perman, UCT, Inc.
This method is used for the determination of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) in fish and seafood: oysters, shrimp, and mussels. Benzo[a]pyrene is the primary analyte of interest. GC–MS instrumentation is used for analysis. |
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Walk-Up Ion Trap in a Multi-User Environment
Stuart Warriner, P. John Wright, and Sebastian Goetz, Bruker Daltonics
Presented here is a case study of a walk-up liquid chromatography ion trap. The client-server software allows users to choose from preprogrammed LC–MS workflows and eliminates the need for tedious method development. Workflows include small molecule quality control to protein identification. |
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High Sensitivity & Resolution with Microflow UHPLC
Khaled Mriziq, Steve Hobbs, Tina Settineri, and Dave Neyer, Eksigent, part of AB SCIEX
This application note describes the use of two new small diameter electrospray ionization electrodes in conjunction with the ExpressHT-ULTRA™ microflow UHPLC system and demonstrates the improved sensitivity and faster separations achievable for high throughput bioanalytical LC–MS-MS. |
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Agilent 1260 Infinity Hybrid SFC/UHPLC System
Martin Vollmer and Markus Becker, Agilent Technologies
The note describes the first solution capable of performing both SFC and UHPLC with a single system thus providing automated orthogonal separation power without any hardware change. |
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NSAIDs on HALO RP-Amide
MAC-MOD Analytical and Advanced Materials Technology
A fast gradient separation of NSAIDs is accomplished in less than 5 min. The HALO RP-Amide phase affords unique selectivity compared to C18 and C8 phases, and provides increased retention for polar analytes that can form hydrogen bonds with the amide functional group. |
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Analysis of Paralytic Shellfish Toxins
Saji George, Maria Ofitserova, and David Mazawa, Pickering Laboratories, Inc.
New official AOAC HPLC method with post-column derivatization and fluorescence detection of paralytic shellfish toxins in bivalve mollusks — OMA 2011.02. |
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A Novel Desalting Column with High Mechanical Strength for Faster Desalting of Proteins
Atis Chakrabarti, Tosoh Bioscience
TSKgel BioAssist DS columns contain spherical 15 µm polyacrylamide particles that are packed in 4.6 mm ID x 15 cm and 10 mm ID x 15 cm PEEK columns. These TSKgel columns are designed for the desalting and buffer exchange of proteins and polynucleotides at analytical (4.6 mm i.d.) and semi-preparative (10 mm i.d.) scales. Either column can be operated safely up to 4 MPa (40 bar or 600 psi). |
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Maximize Zero Grade Air Purity
Reginald J. Bartram, Parker Hannifin Filtration and Separation Division
When running a gas chromatograph (GC) with a flame ionization detector (FID) the choice of purity of the air used is extremely important to the stability of the flame and to your baseline. With the costs of air supplies increasing and a lab desiring to minimize their costs, there is a temptation to use the least expensive air source possible. This paper explores the best purification methods to obtain air for an FID. |
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Molecular Weight Determination of LMWH SEC/MALS vs. SEC/ UV-RI
Wyatt Technology
Low-molecular-weight heparins (LMWHs) are obtained by fractionation or depolymerization of natural heparins. They are defined as having a mass-average molecular weight of less than 8000, for which at least 60% of the total weight has a molecular mass less than 8000. |
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