In our August issue:
COLUMNS
COLUMN WATCH
Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative Applications
K. Koyanagi, I. Sallay, and Ronald E. Majors
Often, little thought is given to the suitability of the base silica for a given assay. Mechanical strength, acid and alkaline durability, loadability, and overload characteristics are a few of the parameters that should be evaluated. |
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LC TROUBLESHOOTING
How Much Retention Time Variation Is Normal?
John W. Dolan
Small changes in retention time with an LC method are normal. At what point is a problem suggested? |
PERSPECTIVES IN MODERN HPLC
Seven Common Faux Pas in Modern HPLC
Michael W. Dong
Because of improved technologies and other changes in modern HPLC, these seven common practices are now highly questionable. Are you committing any of these “faux pas”? |
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THE ESSENTIALS
Modern Mass Spectrometry in Protein Analysis
A brief overview of the analytical approaches used for the analysis of biomolecules |
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PEER-REVIEWED ARTICLES
Translations Between Differing Liquid Chromatography Formats: Advantages, Principles, and Possible Pitfalls
Patrik Petersson, Melvin R. Euerby, and Matthew A. James
When transferring a gradient method between HPLC and UHPLC, various potential pitfalls may result in selectivity differences, changes in efficiency, and failure to meet resolution system suitability criteria. By taking certain precautions, you can ensure smooth translation of your methods. |
Current Practices and Considerations for a Stability-Indicating Method in Pharmaceutical Analysis
Arindam Roy, Anthony Wilken, Joseph Henry, and Luis Collazo-Malave
What are the characteristics and relevant considerations for the development and validation of a stability-indicating method? Best practices described here can guide the development of your method. |
Developments in the Preparation of Organic Polymer Monoliths for the Separation of Small Molecules
Jiri Urban and Pavel Jandera
Recent developments have made organic polymer monoliths much more suitable for the separation of small molecules. |
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