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In our June issue:
COLUMNS
Column Watch
Making the Most of Size-Exclusion Bioseparations Using Sub-2-μm Column Technology
Te-Wei Chu, Veronica Qin, Andrew Coffey, Eric Lyster, Wu Chen, and Gregory Staples
With SEC, special care is required to achieve in practice the chromatographic efficiency that is expected from theory.
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LC Troubleshooting
Mixing and Mixers in Liquid Chromatography, Part III: Solutions for Problems with Sample Diluents
Zachary Breitbach, Corianne Randstrom, Jean Chang, Michael Lesslie,
Gregory Webster, and Dwight R. Stoll
An in-line mixer between the sample injector and column may resolve problems with peak shape caused during sample dilution.
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Improving Confidence and Productivity in Released Glycan Analysis for Biotherapeutic Development
Wednesday, July 17, 2019 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST
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Perspectives in Modern HPLC
Modern HPLC Pumps: Perspectives, Principles, and Practices
Konstantin Shoykhet, Ken Broeckhoven, and Michael W. Dong
Gaining a solid understanding of how HPLC instrumentation works will help you achieve success in the analytical laboratory. This four-part series is here to guide you, starting with pumps.
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Ensuring GxP Data is Available and Enduring Throughout its Retention Period
Thursday, June 27, 2019 at 10am EDT | 9am CDT | 3pm BST | 4pm CEST
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Focus on Biopharmaceutical Analysis
Practical Considerations of Capillary Electrophoresis–Mass Spectrometry for Analysis of Biotherapeutics
Anurag S. Rathore, Ramesh Kumar, and Ira S. Krull
Coupling CE with MS presents some challenges. Here, we discuss the advantages of CE–MS over LC–MS, and the parameters that are important to obtain a stable CE–MS profile.
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The Essentials
Solving Recovery Problems in Solid-Phase Extraction
Improved practices in chemistry selection and sample processing may resolve recovery issues related to target analytes or internal standards.
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Feature Articles
Data Integrity Focus, Part VI: Who Is Looking Over Your Shoulder? Quality Oversight for Data Integrity
R.D. McDowall
What are the roles and responsibilities of quality assurance staff for ensuring data integrity in an organization?
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Evaluation of Achiral Stationary Phases for Gradient Screening withSupercritical Fluid Chromatography
Raffeal Bennett, Matthew Przybyciel, Erik L. Regalado, and Mirlinda Biba
Unlike reversed-phase liquid chromatography, SFC lacks a universal stationary phase. Thus, it is important to re-evaluate the default column screening library used with SFC. In this study, three uncommon achiral SFC columns were investigated and compared to three popular stationary phases.
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THE APPLICATION NOTEBOOK
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Recent Developments in HPLC and UHPLC
Vacuum-Jacketed Columns: Maximum Efficiency, Easy Deployment Without Oven,
and Improved LC–MS Performance
Fabrice Gritti
The design of a user-friendly vacuum-jacketed column (VJC) is described for improved LC–MS performance, which does not require a large vacuum chamber with multiple vacuum pumps. Using this configuration, the experimental peak capacities measured for a 2.1 mm x 100 mm column packed with sub-2-μm particles and placed in the VJC-MS probe are doubled with respect to standard LC–MS systems.
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Recycle Reversed-Phase Liquid Chromatography to Achieve Separations Based on One H/D Substitution on Aromatic Hydrocarbons
Kazuhiro Kimata, Tsunehisa Hirose, Eisuke Kanao, Takuya Kubo, Koji Otsuka, Ken Hosoya, Kohei Yoshikawa, Eiichiro Fukusaki, and Nobuo Tanaka
Ultrahigh efficiency separations based on the presence of one deuterium in benzene, toluene, and naphthalene were achieved by recycle chromatography using C18 silica columns. The discrimination mechanism of H/D isotopic species is discussed based on the dispersion interactions of a CH/CD group of the solute with the stationary phase as well as the mobile phase.
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Progress in Peak Processing
M. Farooq Wahab, Garrett Hellinghausen, and Daniel W. Armstrong
The advantages and limitations of several recently introduced mathematical procedures for enhancing peak resolution in liquid chromatography (LC) are described. Despite advanced separation technologies and extensive knowledge in method development, peak overlap is still commonly observed. This article gives a brief overview of the advantages and limitations of recently introduced mathematical procedures for enhancing resolution.
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Optimization of MS-Compatible Mobile Phases for IEX Separation of Monoclonal Antibodies
Evelin Farsang, Amarande Murisier, Krisztián Horváth, Olivier Colas, Alain Beck, Davy Guillarme, and Szabolcs Fekete
The impact of ionic strength, buffer capacity, and pH-response on the retention behavior and peak shape of mAb species characterization is evaluated for IEX-MS. The aim of the present study was to understand the impact of ionic strength, buffer capacity, and pH-response on the retention behavior and peak shape of mAb species.
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| LIVE WEBCASTS |
Better Insights into Charge Variants of Therapeutic IgGs
Friday, June 21, 2019 at 11 am EDT | 8 am PDT | 4 pm BST | 5 pm CEST
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Editors' Series Sample Preparation Methods for Ensuring Quality Control in LC–MS–based Metabolomics
Monday, June 24, 2019 at 11 am EDT | 8 am PDT | 4 pm BST | 5 pm CEST
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Optimize Your GC Lab with On-Site Hydrogen Generation
Tuesday, June 25, 2019 at 2 pm EDT | 1 pm CDT | 11 am PDT
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Fundamentals of ICP-MS
Tuesday, Jun. 25, 2019 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST
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Filtration in Dissolution Testing
Friday, June 28, 2019 at 11 am EDT | 8 am PDT | 4 pm BST | 5 pm CEST
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| ON DEMAND WEBCASTS |
Analysis of Underivatized Biogenic Amines by LC–MS/MS Using an Alternative Ion-pairing Chromatography Technique
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Editors' Series: Water, Water Everywhere: Good Practice with Water in the Analytical Lab
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Application of New Mass Spectrometric Methods and Workflows to Determine Authenticity of Food
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HPLC Method Development: Practical Approaches for Identifying Equivalent and Orthogonal Columns
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Increased Confidence in Untargeted Volatile E&L Analysis by Static Headspace Sampling Coupled to HRAM GC–MS
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Overcoming Common Challenges in Waste and Surface Waters Analysis
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Strategies for Profiling Screening, Characterization and Quantitation of N-Linked Glycans
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Transforming High Performance LC–MS Analysis in Biopharma: From Molecular Characterization to Attribute Monitoring
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Applying High Resolution and High Efficiency Size Exclusion Chromatography Technology to Biotherapeutic Characterization
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Latest Advancements in Absolute Characterization of Proteins, Polymers and Nanoparticles
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