Direct measurement of pesticides down to parts-per-quadrillion with Waters® ACQUITY UPLC® and Xevo™ TQ-S. This simplifies analysis, enabling a rapid time to result and a very high throughput method.

Miniaturized Nano HPLC for Increased MS Sensitivity
By Evert-Jan Sneekes, Dionex Corporation

This application combines nano HPLC with electrospray (ESI)-MS to demonstrate the use of 20 and 10 µm columns while dropping the flow rate from 200-300 nL/min to as low as 20 nL/min.

GPC cleanup of olive oil samples
By Silvia Marten, KNAUER, Germany

The implementation of an automated sample cleaning method for analyzing pesticide residues in olive oil by HPLC is presented. Pesticides were separated from the oily matrix by gel permeation chromatography according to US EPA SW-846 method 3640A.

Determining saccharidic tracers in atmospheric aerosols
By Silke Rick, Andrea Wille and Alfred Steinbach, Metrohm International Headquarters

Aerosols have a strong impact on the climate. Within the organic aerosol fraction, anhydrosugars and sugar alcohols are valuable tracers for biomass burning and biogenic sources. They are best determined by ion chromatography coupled to pulsed amperometric detection. Sample preparation merely involves a simple water extraction. Detection limits are in the ng/m³ range.

LC/MS Method for Identification of Illicit Drug Salt Forms
By Guifeng Jiang, Terry Zhang, Kathryn Preston, Thermo Fisher Scientific

This application note describes an accurate and robust LC/MS method developed for the identification of illicit drug salt forms. Cocaine,amphetamine,methamphetamine and MDMA are often encountered in the form of salts. The method utilizes two independent parameters – retention time and mass spectral signature – to establish the identity of drug salt forms.

Flash Purification of d-tocopherol & Subsequent HPLC Analysis for Sample Purity
By L Lloyd, S Ball and K Mapp, Varian, Inc

Two options now exist for purifications of crude samples. HPLC is suited to the purification of complex samples where high purity is required. Flash is suited to less complex samples or where lower purities are acceptable.

Overcoming Compound Volatility with Sub-ambient ELSD
By Stephen Bullock, Varian, Inc

In the past, quantification and purity measurements using evaporative light scattering detection showed poor accuracy due to compound volatility and response variation across a solvent gradient. However, the Varian 385-LC increases the accuracy of measurement by minimizing compound volatility effects.

Glycan Analysis using the ACQUITY UPLC H-Class Bio System
By Vera B. Ivleva, Martin Gilar, Tom Wheat, Waters Corporation

Robust analyses of the glycans of glycoproteins can be readily developed with the ACQUITY UPLC H-Class Bio System featuring AutoBlend Plus Technology and Glycan Separation Technology Columns.

Analytical HPLC to Preparative HPLC: Scale-Up Techniques using a Natural Product Extract
By Andrew Aubin and Ronan Cleary, Waters Corporation

This application note demonstrates that analytical chromatography can be successfully scaled to preparatory chromatography easily by using a systematic approach. Also demonstrated is the unique capability of the Waters AutoPurification System, which allows users to perform both analytical and preparatory chromatography on the same system with no performance compromise.

Analysis of Veterinary Drugs Using ACQUITY UPLC CSH Columns
Waters Corporation

This application note demonstrates the use of ACQUITY UPLC CSH columns for the fast separation and detection of 9 veterinary drugs.

Heparin analysis and characterization
James Knobloch and Patrick Shaklee, Wyatt Technology Corporation

The U.S. Food and Drug Administration (FDA) has scrutinized LMW-heparin products closely due to their inherent variability and polydispersity. Previously, these materials had to be characterized by relative techniques involving viscometry or standard elution-based chromatographic methods.

With the introduction of Antec’s ROXY™ EC system, the synthesis of metabolites as well as the mimicking of phase I and II metabolism has become possible in routine within a few minutes.

This application demonstrates the determination of aucubin, genipoide, and ponoresinol diglucoside in cortex eucommiae using accelerated solvent extraction and HPLC. It is an excellent example of Dionex solutions for natural products analysis.