Sorptive extraction with TD-GC/TOF-MS analysis was used to characterise a wide range of volatile organic compounds found in beer. The classical EI-spectra and high sensitivity provided by TOF-MS ensured the accurate identification of both high concentration and trace-level compounds within such a complex matrix.

Combining Hardware, Software, and Chromatography to Improve the GC/MS Analysis of Semi-Volatile Compounds
By Jessie Butler, David Steiniger, Trisa Robarge, Eric Phillips, Thermo Fisher Scientific

A method for analysis of semi-volatile organic compounds by GC/MS according to published quality control and method guidelines is described resulting in shorter run times and increased overall throughput.

PNIPAM gels exhibit negative thermosensitivity, they undergo shrinkage with increasing temperature. Precise control over a hydrogel’s shrinking and swelling enables control over its drug release/uptake properties. The molecular weight distributions of PNIPAM polymers can be accurately measured by GPC.

Enhanced Peptide Identification with an Ultra High Resolution Mass Spectrometer
By Laura C. Main, Ralf Ketterlinus, Bruker Daltonik GmbH

Analysis of biological samples frequently involves the identification of peptides from low amounts of complex samples. Confident identification of these peptides requires rapid generation of high quality, high sensitivity MS and MSMS data. The maXis^TM TOF mass spectrometer incorporates novel technical innovations, which together produce an unprecedented level of data quality, resulting in a significant increase in the number of peptides identified from challenging samples.

Characterization Studies of PEGylated Lysozyme
By W. Conze & A. Moosmann, Tosoh Bioscience

PEGylation is a common method to prolong serum half-life of therapeutic proteins. This application note describes the use of size exclusion and ion exchange chromatography for the characterization of PEGylated proteins.

Is there really a difference between Flash and HPLC for LC Purification?
By L Lloyd, S Ball and K Mapp, Varian, Inc

A wide range of instruments, columns and media are available for LC purification, ranging from flash to HPLC. This application note examines the advantages and disadvantages of using these techniques for compound purification.

Analysis of Biomolecules by Size-Exclusion UltraPerformance Liquid Chromatography
By Kenneth J. Fountain, Paula Hong, Susan Serpa, Edouard S. P. Bouvier, Damian Morrison, Waters Corporation

This application note will demonstrate the use of the new ACQUITY UPLC SEC solution for the improved detection and/or faster analysis of protein aggregates in biopharmaceuticals.

Fast Screening for Vitamins and Dyes in Health Beverages
By Mark Benvenuti, Waters Corporation

A rapid and robust method for the analysis of water-soluble vitamins and food dyes in health beverages. The ACQUITY UPLC H-Class System with PDA detection allows for the analysis of multiple analytes using a straightforward workflow that is a reliable alternative to lengthy HPLC separations.

Reliability of Size Exclusion Chromatography Measurements on ACQUITY UPLC H-Class Bio System
By Paula Hong, Waters Corporation

The ACQUITY UPLC H-Class Bio System along with an ACQUITY UPLC BEH200 SEC Column deliver reliable and reproducible SEC separations for biomolocules.

Light Scattering for the Masses™
By Wyatt Technology Corporation

Hyaluronic Acid analyses showing marked differences among different samples.

A Rapid iMethod™ Test for the Screening of Antibiotics in Animal Products
AB SCIEX

This Application Note outlines the instrument requirements and expected results obtainable from the AB Sciex iMethodTM Test for Antibiotic Screening when using a 4000 QTRAP® LC/MS/MS System

The article describes a highly efficient combustion ion chromatography system (CIC) that is used for the simultaneous trace-level determination of halogens and sulfur in liquid and solid combustible samples. Results obtained from the analysis of a polymer standard as well as a S-benzyl thiouronium chloride and four different fuel samples are presented.