COLUMNS
COLUMN WATCH
New Chromatography Columns and Accessories at Pittcon 2011: Part I
Ronald E. Majors
A review of the liquid chromatography columns and accessories for analytical and preparative chromatography unveiled at Pittcon 2011

ADVERTISEMENT AGILENT COMPLIANCE—CLEARLY BETTER AGAIN Agilent has been ranked number one in Compliance Services five consecutive times since 1995, in an independent customer survey. Why is Agilent Clearly Better Again? Better Science: Agilent provides fully automated, metrology-based qualification services you can trust. When you need virtually audit-proof analytical instrument qualification, choose the leader. Choose Agilent. Learn more and get a FREE white paper and qualification primer.

LC TROUBLESHOOTING
Selectivity in Reversed-Phase LC Separations, Part III: Column-Type Selectivity
John W. Dolan
Comparisons between different C18 columns and the best way to select which one to use

GC CONNECTIONS
Valves for Gas Chromatography: Fundamentals
John V. Hinshaw
The first installment in this series on valves presents basic concepts, applications, and implementations.

BIOTECHNOLOGY TODAY
High-Throughput Tools and Approaches for Development of Process Chromatography Steps
Anurag S. Rathore, Rahul Bhambure, and Ira S. Krull
An overview of high-throughput process development (HTPD) tools and approaches, as well as a case study

ARTICLES

The 2011 LCGC Pittcon Awards
Laura Bush
The winners of LCGC’s fourth annual awards, who will be honored at Pittcon 2011 and interviewed in the LCGC Pittcon Theater.

HILIC–MS Sensitivity without Silica
Luisa Pereira
What challenges occur when using hydrophilic interaction liquid chromatography (HILIC), and what options are available for analysis?

The Secrets of Electrospray Ionization: Why Less is More
An interview with Kevin Schug of the University of Texas at Arlington.

WEBCASTS

Exploring Recent Developments in UHPLC Technology for Efficient System Operation and Optimal Results
LIVE WEBCAST: Thursday, March 10, 2011 from 11:00 AM - 12:00 PM EST
In the upcoming webinar, Exploring Recent Developments in UHPLC Technology for Efficient System Operation and Optimal Results, you are invited to learn about the latest advancements in Ultra High Performance Liquid Chromatography (UHPLC) and hybrid HPLC/UHPLC systems. The benefits offered by the use of the patented Force Feedback Control (FFC) technology in these systems will be discussed along with best practice recommendations that will help users make the most efficient use of their UHPLC systems.
» Register Free

Improving Efficiency in High Sample-Volume Laboratories Using Turbulent Flow Chromatography & LC Multiplexing
LIVE WEBCAST: Wednesday, March 23, 2011 from 10:00 AM – 11:00 AM EST
This webinar will introduce the latest solutions for gaining efficiency in high-throughput food testing laboratory environments such as contract testing labs. Dramatically increase productivity through turbulent flow chromatography, a superior online sample extraction technique for complex matrices. This innovative approach can be used standalone or it can be coupled with LC multiplexing to further increase efficiency and lower costs. Learn how food testing labs are implementing these new technologies to reduce cost per sample and improve ROI.
» Register Free

Maximum Power and Confidence for Screening and Qual/Quan Analysis with High-Res, Accurate Mass LC-MS with Orbitrap Technology
LIVE WEBCAST: Tuesday, March 29, 2011 at 8:00 AM PST; 11:00 AM EST
This webinar is intended to exemplify the power of high resolution accurate mass (HRAM) technology of compound identification, screening and quantitative analysis for clinical research, environmental, food safety and pharmaceutical applications.
» Register Free

Optimizing Carbohydrate, Glycan and Sialic Acid Analysis Using HPAE-PAD
LIVE WEBCAST: Wednesday, March 30, 2011 at 11:00 AM ET
High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) has been widely used to characterize carbohydrates because the method allows rapid and direct quantification of underivatized samples. HPAE-PAD not only separates oligosaccharides according to charge, but can also resolve oligosaccharides with the same charge according to size, sugar composition, and linkage of monosaccharide units.
» Register Free

On-Line SPE-UHPLC for High Sensitivity/High Speed Chromatography Analysis
LIVE WEBCAST: Thursday, March 31, 2011 at 8:00 AM PDT; 11:00 AM EDT; 15:00 GMT
Sample preparation is typically required for most chromatography applications. Among the techniques of interest, solid phase extraction (SPE) is very wide spread across all applications and samples. Although a powerful technique, conventional SPE is a fully manual process – making it error prone, requiring manual documentation, and raising safety issues when using hazardous samples. Another limitation is that it is difficult to keep pace with the increased throughput offered by UHPLC – even when using parallel sample preparation.
» Register Free

Empower 3 Chromatography Software: New Tools and Calculation Capabilities to Enhance Productivity
ON DEMAND WEBCAST
Waters^® Empower™ 3 is the latest version of Waters’ chromatography software suite that provides several usability and workflow enhancements that will help improve laboratory productivity. Attendees of this webinar will learn about key features and benefits of Empower 3.
» Register Free

Implementing Ultra-high-pressure LC (UHPLC): Perspective, Performance, Practice and Potential Issues
ON DEMAND WEBCAST
This webinar provides an overview on how to implement UHPLC in your laboratory for research and quality control applications. Topics will include an introduction to the technology, performance enhancements, its impacts on method development and sample analysis, implementation strategies, potential issues and how to mitigate operational nuances and pitfalls. Case studies are drawn from pharmaceutical analysis examples to illustrate benefits and best practice of UHPLC.
» Register Free

The CHROMacademy Essential Guide to Electrospray Ionization (ESI) for LC-MS (Part 1) Understanding Electrospray Ionisation (ESI) for LC-MS
ON DEMAND WEBCAST

• Overview of ESI Hardware
• When to use electrospray
• Eluents and eluent flow rate
• The nebulization process – key factors in optimizing instrument response
• Desolvation processes - ensuring efficient droplet desolvation
• Mechanisms for producing ions in the gas phase – optimizing sensitivity
• Quantitative aspects of ESI – droplet and gas phase processes leading to ion suppression / enhancement